PURIFIED THERMOSTABLE ENDONUCLEASE

摘要

The present invention discloses a substantially purified and isolated form of a native or recombinant thermostable class II AP endonuclease. In particular, the invention relates to an AP endonuclease of a thermophilic bacteria and to a method for cloning and expressing the recombinant form of the enzyme in a host cell such as Escherichia coli. The AP endonuclease of the present invention remains stable when subjected to elevated temperatures for the time necessary to effect denaturation of double-stranded nucleic acids. The recombinant thermostable endonuclease is useful in a ligase chain reaction.