Molecular cloning of the genes reponsible for collagenase production from clostridium histolyticum

摘要

Genetically-engineered (E. coli) carrying vectors containing an insert that codes for non-native and native forms of (Clostridium histolyticum) collagenase. The recombinant DNA segments coding for collagenase are expressed efficiently in the transformed cells to yield enzymatically active and immunologically cross-reactive collagenase. Genetically-engineered collagenase is used for isolation of pancreatic islets, for isolation of endothelial cells, for the isolation of dispersed tumor cells, or for the treatment of "slipped disc".