INKJET DEPOSITION OF REAGENTS FOR HISTOLOGICAL SAMPLES

摘要

Devices and methods for the deposition of reagent droplets onto cells or tissue samples are disclosed. Optionally the samples have a protective fluid layer, the droplets have volumes between about 1 pL and about 50 pL, a pH modifier is dispensed to the samples, the droplets have a velocity of between about 5 m/s to about 15 m/s, the droplets have a kinetic energy of greater than 9.52x10-10 Joules, and the spatial density of reagents deposited on the samples ranges from about 50 dpi to about 1200 dpi. Also disclosed are reagent compositions suitable for dispensing via a droplet-on-demand system, whereby the reagent composition is selected from the group consisting of a primary stain reagent composition, an antibody reagent composition and a large molecule staining composition. Also an inkjet deposition system and means for imaging a slide containing a sample are disclosed.