COMPOSITIONS AND METHODS FOR HIGH THROUGHPUT PROTEIN SEQUENCING

摘要

Provided are compositions and methods for elucidating the amino acid sequences of a plurality of polypeptides in high-throughput approaches. The compositions include unique binding partners that have specificity for N-terminal amino acids. The methods include using the unique binding partners in processes that involves obtaining a plurality of polypeptides, binding detectably labeled N-terminal amino acid binding agents to the N-terminal amino acid of the polypeptides, detecting the N-terminal amino acid binding agents to identify the N-terminal amino acid for some or all of the polypeptides, removing the N-terminal amino acid binding agents, liberating the N-terminal amino acid to reveal the next amino acid in the polypeptides in the N->C terminal direction, and repeating the process to determine some or all of the amino acid sequence of the polypeptides. The approaches be used to analyze polypeptides from a wide diversity of sources, and for detecting proteins of low abundance from any particular source.