发明名称 |
AGROCYBE AEGERITA LECTIN AAL-2, AND ENCODING GENE THEREOF, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF |
摘要 |
The present invention provides the Agrocybe aegerita lectin AAL-2, its encoding gene, the preparation methods and its uses. The invention uses the affinity chromatography separated the lectin protein AAL-2 from the Agrocybe aegerita total protein, of which the amino acid sequence is set forth in SEQ ID NO: 2 and the nucleotide sequence encoding the said protein is set forth in SEQ ID NO: 1. Cell tests indicate that the Agrocybe aegerita lectin AAL-2 isolated in the invention has excellent inhibition activity to the tumor cells, which can induce apoptosis of hepatoma cells, significantly. Animal experiments show that AAL-2 has a better therapeutic effect on tumor. The glycochip technology test results show that the AAL-2 preferentially binds to the carbohydrate or glycoproteins with the N-Acetylglucosamine as the terminal, which can be used as a reagent for testing the N-Acetylglucosamine relevant diseases or the N-Acetylglucosamine relevant carbohydrate structure. |
申请公布号 |
EP2740739(B1) |
申请公布日期 |
2016.12.28 |
申请号 |
EP20110870457 |
申请日期 |
2011.10.24 |
申请人 |
Wuhan Alpha-Omega Biology Technology Co., Ltd. |
发明人 |
SUN, Hui;JIANG, Shuai;GU, Bianli;CHEN, Yijie;YU, Guojun;YIN, Yalin;ZHANG, Jinlin |
分类号 |
C07K14/375;A61K38/16;A61P35/00;C07K1/22;C12N1/00;C12N15/31;C12N15/63;G01N33/50 |
主分类号 |
C07K14/375 |
代理机构 |
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代理人 |
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地址 |
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