PULSED LASER AND BIOANALYTIC SYSTEM

摘要

Apparatus and methods for producing ultrashort optical pulses are described. A high-power, solid-state, passively mode-locked laser can be manufactured in a compact module that can be incorporated into a portable instrument for biological or chemical analyses. The pulsed laser may produce sub-100-ps optical pulses at a repetition rate commensurate with electronic data-acquisition rates. The optical pulses may excite samples in reaction chambers of the instrument, and be used to generate a reference clock for operating signal-acquisition and signal-processing electronics of the instrument.

主权项

1. A system for identifying different nucleotides and/or nucleotide analogs, the system comprising:
an optical source configured to produce optical excitation pulses having a single characteristic wavelength; a bio-optoelectronic chip comprising a plurality of reaction chambers each with a time-binning photodetector at each reaction chamber; a receptacle configured to receive the bio-optoelectronic chip; and optical components arranged to couple the optical excitation pulses to the bio-optoelectronic chip, wherein the bio-optoelectronic chip is configured to generate electronic signals comprising three different electronic signals from each time-binning photodetector that distinguish three different species from among the nucleotides and/or nucleotide analogs in response to excitation of fluorophores with radiation from the optical excitation pulses having the single characteristic wavelength, wherein the fluorophores are linked to the three different species.