SELF-ASSEMBLING TWO-DIMENSIONAL PROTEIN ARRAYS

摘要

This document relates to two dimensional (2D) protein arrays can be used in biotechnology applications, as well as methods of making and using 2D protein arrays. In some cases, a 2D protein array can be used to evaluate (e.g., image) a structure (e.g., a three dimensional (3D) structure) of a protein of interest. In some cases, a 2D protein array can be used to evaluate (e.g., characterize) protein-protein interactions (e.g., stable interactions vs. transient interactions). In some cases, a 2D protein array can be used to evaluate a binding domain in a protein of interest. In some cases, a 2D protein array can be used to evaluate (e.g., identify) binding targets and/or partners of a protein of interest.

主权项

1. A two-dimensional (2D) protein array comprising:
a plurality of oligomeric protein unit cells, wherein each oligomeric protein unit cell comprises at least one axis of rotational symmetry, and wherein each oligomeric protein unit cell comprises a plurality of self-assembling proteins; wherein said plurality of oligomeric protein unit cells interact with one another at one or more symmetrically repeated protein-protein interfaces.