細胞を含む支持体層に培養液を灌流させることを特徴とする３次元培養組織

摘要

PROBLEM TO BE SOLVED: To provide in vitro three-dimensional cultured tissue having more resembled status to in vivo tissue.SOLUTION: Three-dimensional cultured tissue in culture medium perfusion method is produced, as it is found that cells can be cultured in imitation of body fluid circulation environment by: adding tensile force to cells like a living body by inhibiting shrinkage in the horizontal direction of a supporting layer containing the cells; and supplying a fresh culture medium always in a constant flow rate. In the three-dimensional cultured tissue, it becomes possible to maintain cells for a prolonged period in a more resembled status to in vivo tissue.