METHOD AND SYSTEM FOR SEQUENCING IN CHARACTERIZATION OF ANTIBODY BINDING BEHAVIOR

摘要

A method and system for characterization of antibody-bound targets by sequencing of synthetic oligonucleotides bound to the antibodies, the method including: receiving a sample having a set of antibodies; conjugating each of the set of antibodies with an oligonucleotide, thereby generating a set of oligonucleotide-conjugated antibodies; binding a first subset of the set of oligonucleotide-conjugated antibodies to a set of targets at a capture substrate; determining a sequence for at least one of: 1) each oligonucleotide of the first subset of oligonucleotide-conjugated antibodies that bind to the set of targets and 2) each oligonucleotide of a second subset of the set of oligonucleotide-conjugated antibodies that fail to bind to the set of targets; and generating an analysis of the sample from the sequences determined from at least one of the first and the second subsets of oligonucleotide-conjugated antibodies.

主权项

1. A method for characterization of antibody-bound targets by sequencing of synthetic oligonucleotides, comprising:
conjugating each of a set of antibodies with a synthetic oligonucleotide, thereby generating a set of oligonucleotide-conjugated antibodies; binding at least a first subset of the set of oligonucleotide-conjugated antibodies to a set of targets at a capture substrate, the set of targets associated with a sample from a subject; determining a sequence for at least one of: 1) each oligonucleotide of the first subset of oligonucleotide-conjugated antibodies that bind to the set of targets and 2) each oligonucleotide of a second subset of the set of oligonucleotide-conjugated antibodies that fail to bind to the set of targets; and generating an analysis of the sample from the sequences determined from at least one of the first and the second subsets of oligonucleotide-conjugated antibodies, wherein the analysis identifies the set of targets based upon sequencing of each oligonucleotide of at least on of the first and the second subsets, and wherein the analysis is informative of a relative distribution of binding between antibodies of the set of oligonucleotide-conjugated antibodies and the set of targets.