发明名称 |
ANALYSIS DEVICES, KITS, AND RELATED METHODS FOR DIGITAL QUANTIFICATION OF NUCLEIC ACIDS AND OTHER ANALYTES |
摘要 |
Provided are devices and methods for effecting processing of samples, including essentially isothermal amplification of nucleic acids, at multiple reaction locations in a single device. In some embodiments, the disclosed devices and methods provide for effecting parallel sample processing in several hundred locations on a single device. |
申请公布号 |
US2016362734(A1) |
申请公布日期 |
2016.12.15 |
申请号 |
US201615247795 |
申请日期 |
2016.08.25 |
申请人 |
California Institute of Technology ;University of Chicago |
发明人 |
Ismagilov Rustem F.;Shen Feng;Kreutz Jason E.;Sun Bing;Du Wenbin |
分类号 |
C12Q1/68;B01L3/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A method of amplifying a nucleic acid molecule, comprising:
contacting (a) a sample comprising at least one nucleic acid molecule disposed at a plurality of first areas, with (b) at least one component of an amplification reagent disposed in a plurality of second areas, the contacting being effected by placing the first and second areas into direct fluid communication with one another; and the contacting comprises effecting relative motion between a substrate comprising the first area with a substrate comprising the second area; and exposing the area having the at least one nucleic acid molecule to conditions effective for amplification of the at least one nucleic acid molecule. |
地址 |
Pasadena CA US |