| 摘要 |
In order that, when culture medium replacement such as total replacement of a culture medium or replacement thereof at a predetermined rate is performed in a closed system culture vessel (101) used in an automatic culture device, an old culture medium in the culture vessel is totally discharged or a predetermined quantity thereof is discharged by making a change to a discharge port, a culture vessel (1010) has therein a two-layer-structure culture space, a first vessel (302) is housed in a second vessel (303), and a culture medium is held in a space inside the second vessel and outside the first vessel during culture. The first vessel and the second vessel have first and second ports (306, 307) for supplying a cell suspension or the culture medium thereto, and supplying and discharging air thereto and therefrom, and third and fourth ports (308, 301) for discharging the culture medium therefrom, respectively. The fourth port (301) has a discharge port extending to the lower portion (304) of the first vessel (302) and the peripheral portion (305) of the second vessel (303). |
