发明名称 |
METHODS FOR DETECTING ADENOSINE MONOPHOSPHATE IN BIOLOGICAL SAMPLES |
摘要 |
The present invention provides compositions and methods to detect and/or determine the amount and/or presence of adenosine monophosphate (AMP) in biological samples. The method comprises converting substantially all AMP in the solution to adenosine diphosphate (ADP) using a first enzyme, suitably polyphosphate:AMP phosphotransferase (PAP), that is capable of converting AMP to ADP; converting the ADP in the solution to adenosine triphosphate (ATP) using a second enzyme, suitably adenylate kinase (AK), that is capable of converting ADP to ATP; determining the amount of the ATP produced using a bioluminescent reaction utilizing a luciferase enzyme and a substrate for the luciferase enzyme; and using the amount of ATP produced to determine the amount of AMP present in the original solution. |
申请公布号 |
EP2771480(B1) |
申请公布日期 |
2016.12.14 |
申请号 |
EP20120791022 |
申请日期 |
2012.10.29 |
申请人 |
Promega Corporation |
发明人 |
GOUELI, Said, A.;HSIAO, Kevin;ZEGZOUTI, Hicham |
分类号 |
C12Q1/44;C12Q1/48;G01N33/53 |
主分类号 |
C12Q1/44 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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