摘要 |
The present invention enables accurate detection of the position of a spot on a TLC plate. The spot detection is conducted by: using a TLC plate provided with a separating agent layer, said separating agent layer having a specific optical response, together with a transfer-receiving sheet which is to be superimposed on the separating agent layer and which has a specific porosity for transferring thereonto a spot in the separating agent layer; developing a target substance in the separating agent layer of the TLC plate; superimposing the transfer-receiving sheet on the separating agent layer at least in the part wherein the target substance has been developed; moistening the separating agent layer and the transfer-receiving sheet thus superimposed together with a solvent for transfer; vaporizing the solvent for transfer from the side of the transfer-receiving sheet superimposed on the separating agent layer to thereby transfer the spot in the separating agent layer onto the transfer-receiving sheet; and then optically detecting the spot having been transferred onto the transfer-receiving sheet. |