Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.
申请公布号
WO2016196261(A1)
申请公布日期
2016.12.08
申请号
WO2016US34570
申请日期
2016.05.27
申请人
BOEHRINGER INGELHEIM INTERNATIONAL GMBH;HILLER, Gregory Walter;GAGNON, Matthew Paul;OVALLE, Ana Maria
发明人
HILLER, Gregory Walter;GAGNON, Matthew Paul;OVALLE, Ana Maria