发明名称 |
SYSTEM AND METHODS FOR DETECTING RIBOSOME INHIBITION |
摘要 |
This disclosure describes a cell genetically modified to detect ribosome inhibition in the cell and methods involving such a cell. Generally, the genetically-modified cell includes an aminoglycoside-sensitive orthogonal 16S rRNA (O-16S) coding region bearing a mutated anti- Shine-Dalgarno (O-ASD) sequence, a repressor/operator system, and a polynucleotide encoding a detectable reporter under transcriptional control of the repressor/operator system. The repressor/operator system includes a coding region that encodes a transcriptional regulator and having an orthogonal SD (O-SD) sequence complementary to the 16S rRNA O-ASD sequence. The operator sequence, which is repressable by the transcriptional regulator, is operably linked to the polynucleotide encoding a detectable reporter. |
申请公布号 |
WO2016196749(A1) |
申请公布日期 |
2016.12.08 |
申请号 |
WO2016US35451 |
申请日期 |
2016.06.02 |
申请人 |
STC.UNM;MELANCON, Charles;HUANG, Shijie |
发明人 |
MELANCON, Charles;HUANG, Shijie |
分类号 |
C12N5/10;C12N15/11;C12Q1/00 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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