L-arabinose isomerase variants with improved conversion activity and method for production of D-tagatose using them

摘要

The present invention relates to the development of an L-arabinose isomerase variant from Thermotoga neapolitana DSM 5068, which is a kind of thermophile, on the basis of protein molecular modeling. Moreover, the present invention relates to a method of producing D-tagatose from D-galactose by using the enzyme or a microorganism of the genus Corynebacterium expressing the enzyme.

主权项

1. An arabinose isomerase variant having an increased activity of converting D-galactose into D-tagatose, the arabinose isomerase variant having a substitution of an amino acid other than phenylalanine for an amino acid at position 275 and a substitution of proline for an amino acid at position 469 of the amino acid sequence set forth in SEQ ID NO: 1.