| 摘要 |
Devices and methods for detecting, identifying, and sequencing, compounds, complexes, and molecules are described. Electronic detection is combined with optical excitation to determine the presence or identity of an analyte of interest. Embodiments of the invention additionally provide devices and methods that allow highly parallel nucleic acid sequence determination. |
| 主权项 |
1. A device comprising,
a substrate, having a surface, which comprises an integrated circuit chip, an enzyme coupled to the substrate, a photosensitizer coupled to a nucleotide and to the enzyme, a DNA molecule coupled to the enzyme, an array of sensors, on the substrate surface and including a first sensor that is coupled to the enzyme, which are coupled to the electronics of and individually addressable through the integrated circuit chip, and at least four antennas on the substrate surface, adjacent the first sensor, and oriented to localize light that illuminates the substrate surface towards the first sensor; wherein the photosensitizer is configured to communicate electrons between the photosensitizer and at least one of an electron receptor and an electron donor in response to: (a) the photosensitizer and the DNA molecule both coupling to the enzyme, and (b) the photosensitizer being exposed to localized light from the at least four antennas; wherein the first sensor is configured to sense the electrons communicated with the photosensitizer to indicate the nucleotide is coupled to the DNA molecule. |
