Measurement of brain CDP-diacylglycerol synthase 1 enzyme and uses thereof

摘要

Provided herein are methods of diagnosing depressive disorders in a subject by comparing a CDP-diacylglycerol synthase 1 enzyme activity or expression level to enzyme activity or expression level of CDP-diacylglycerol synthase 1 in a control subject. Also herein are methods of predicting therapeutic efficacy of an antidepressant drug in a subject with depressive disorders by monitoring enzyme activity or expression level of CDP-diacylglycerol synthase 1 in the subject. Further provided herein are methods of identifying a compound effective to treat or alleviate the symptoms of depression by monitoring enzyme activity or expression level of CDP-diacylglycerol synthase 1 in a tissue. Further provided are kits for diagnosing depressive disorders.

主权项

1. A method of assessing and monitoring a depressive disorder in a test subject, comprising:
obtaining a sample from the test subject and from a control subject; measuring an expression level of a CDP-diacylglycerol synthase 1 enzyme and of a CDP-diacylglycerol synthase 2 enzyme in the test sample and in the control sample; determining a ratio of the expression levels of the CDP-diacylglycerol synthase 1 enzyme to the CDP-diacylglycerol synthase 2 enzyme in the test sample and in the control sample; comparing the ratio of the expression levels in the test sample to the ratio of the expression levels in the control sample subject; assessing the test subject as having the depressive disorder when a statistically lower ratio is present in the test sample compared to the control sample; and monitoring said ratio in the test subject after an administration of an antidepressant drug to the test subject.