Determination of tissue oxygenation in vivo

摘要

A system and method for noninvasively determining the oxygenation of a tissue, for example, a muscle, in vivo uses optical methods to optically interrogate the tissue in both a visible wavelength range and a near infrared (NIR) wavelength range. The illuminating light is sculpted in intensity to approximately match the absorbance spectrum, for example, with the visible light having an intensity an order of magnitude greater than the NIR light. Training data is obtained from healthy patients in both the visible and NIR ranges simultaneously and used to calculate muscle oxygenation.

主权项

1. A method for generating a set of training data that is useful for determining tissue oxygenation in vivo, comprising:
(a) providing a spectrometer comprising a probe having:
(i) a first light source operable to emit light in a near infrared spectrum having a first radiant flux,(ii) a second light source operable to emit light in a visible spectrum having a second radiant flux that is greater than the first radiant flux, and(iii) a light detector; (b) for a subject, placing the probe such that the light detector detects absorbance spectra of light reflected from or transmitted through a tissue of the subject, the detected absorbance spectra resulting from light emitted by the first and second light sources; (c) using the detected absorbance spectra to calculate a sum of a concentration of oxymyoglobin and a concentration of oxyhemoglobin in the tissue [oxyMbHb]; (d) using the detected absorbance spectrum to calculate a sum of a concentration of deoxymyoglobin and a concentration of deoxyhemoglobin in the tissue [deoxyMbHb]; (e) calculating tissue oxygenations from [oxyMbHb] and [deoxyMbHb]; (f) storing on a computer readable media:
(i) data calculated from the detected absorbance spectra, and(ii) the calculated tissue oxygenation; and (g) repeating steps (b)-(f) for a plurality of subjects to generate a set of training data.