Method for varying the scanning field of a laser scanning microscope

摘要

Disclosed is a method for varying the size of the scanning field of a multifocal laser scanning microscope, said scanning field being scanned in X columns and Y lines, and n laser spots being arranged at a distance d from one another in the scanning field along the slow scanning axis in the sample plane, the distance between the scanned lines in the sample plane being a=d/K, where KεN, the size of the scanning field being varied by varying K. After scanning K lines, a vertical skip is made, e.g. a skip of (n−1)×K+1 lines in the scanning direction or (n+1)×K−1 lines against the scanning direction until at least Y lines have been scanned.

主权项

1. A method of scanning a scanning field of a multifocal laser scanning microscope having a slower scanner and a faster scanner, the scanning field having X columns and Y lines, wherein X and Y are integers greater than 0, comprising:
disposing N laser spots in the scanning field along a scanning direction of the slower scanner, wherein the laser spots have a spacing of D between each other and each laser spot has an associated raster area comprising K adjacent lines, wherein N is greater than 0 and K is greater than 1; determining a spacing A of the K lines in each associated raster area such that A=D/K simultaneously scanning the laser spots across the associated raster areas; moving the laser spots along or against the scanning direction of the slower laser by a skip distance, thereby associating a different raster area with each laser spot; and repeating above steps until at least Y lines have been scanned in total.