发明名称 |
FREMGANGSMÅDE TIL IDENTIFIKATION AF ANTIBIOTISKE MÅL |
摘要 |
Disclosed is a method for identifying an essential gene which serves as an antibiotic target in a bacterium, the method comprising the steps of: (a) generating a pool of mutant bacteria by transposon mutagenesis with an activating transposon (TnA), wherein the TnA comprises a promoter such that transposon insertion into bacterial DNA increases the transcription of a gene at or near the insertion site; (b) growing bacteria from the mutant pool in the presence of different amounts of said antibiotic to produce two or more test cultures; and (c) comparing the distribution of TnA insertions between test cultures to identify a putative essential gene serving as a target of said antibiotic in said bacterium. |
申请公布号 |
DK2705148(T5) |
申请公布日期 |
2017.03.13 |
申请号 |
DK20120723223T |
申请日期 |
2012.05.03 |
申请人 |
Discuva Limited |
发明人 |
WILLIAMS, David, Hugh;TURNER, Arthur, Keith |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|