SHEET ILLUMINATION MICROSCOPE SYSTEM, IMAGE PROCESSING DEVICE AND SHEET ILLUMINATION MICROSCOPY

摘要

An image processing device includes a spatial frequency filtering process unit. The spatial frequency filtering process unit performs a spatial frequency filtering process on image data of a three-dimensional image of the observed object in accordance with the optical transfer characteristic of the microscope apparatus. The spatial frequency filtering process is a process of changing a spatial frequency characteristic included in the three-dimensional image. The three-dimensional image is constructed from a plurality of two-dimensional images of the observed object acquired by the microscope apparatus under a first illumination light amount distribution. The first illumination light amount distribution is formed by moving a condensing position of a light sheet in an illumination direction. The light sheet is emitted to the observed object from the illumination direction.

主权项

1. A sheet illumination microscope system comprising:
a microscope apparatus configured to acquire a two-dimensional image of an observed object under a first illumination light amount distribution, the first illumination light amount distribution being formed by moving a condensing position of a light sheet in an illumination direction orthogonal to an observation direction, and the light sheet being emitted to the observed object from the illumination direction; and an image processing device configured to perform a spatial frequency filtering process on image data of a three-dimensional image of the observed object in accordance with an optical transfer characteristic of the microscope apparatus, the spatial frequency filtering process being a process of changing a spatial frequency characteristic included in the three-dimensional image, and the three-dimensional image being constructed from a plurality of two-dimensional images acquired by the microscope apparatus.