THROMBOLYTIC ENZYME AND METHOD OF OBTAINING SAME

摘要

A thrombolytic enzyme capable of hydrolyzing exo-and endo - epsilon - ( gamma - Glu) - Lys - isopeptide bonds in synthetic substrates, in stabilized fibrin and in its D-D-fragment with a partial amino acid sequence with a structure of a corresponding thereto gene and two additional genes of that same family, with residues of glycine in positions 15, 21, 24, 28, 33, 46, 49, 50 in the primary structure of the protein. A method of preparing a thrombolytic enzyme from medicinal leech salivary gland secretion, or preparations comprising that secretion, including affinity chromatography on antibodies to native enzyme which are immobilized on a solid carrier, subsequent purification of the enzyme by ion-exchange chromatography and gel filtration.