主权项 |
1. A process for the production of a hybridoma, and of a monoclonal antibody or fragments thereof, able to recognize 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 comprising the following steps:
a) immunisation of an animal with a hapten, rendered immunogenic, of formula (1), or a salt thereof, or a derivative thereof in which the carboxylic acid function is protected to form an ester, amide or oxazoline, in which n is an integer between 0 and 3; R2, R3, R4, R5, R6, R7, R8, R9, R10, R11, R14, R15 are independently hydrogen or a C1-4 alkyl, R12 and R13 are a C1-4 alkyl; R is a hydrogen or a substituent selected from the group consisting of acyl, benzyl, alkyl, aryl, alkyl ether, dimethoxytrityl, methoxytrityl, tetrahydropyranyle, triphenylmethyl group, and a silyl derivative; b) sampling of B cells produced by the animal and fusion of the same with myeloma cells to form the hybridoma; c) production, from at least part of the obtained hybridoma, of a monoclonal antibody, or fragments thereof, able to recognize 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3. |