发明名称 |
Methods and compositions related to modulators of eukaryotic cells |
摘要 |
Methods for Identifying protein modulators of eukaryotic cells by expressing a combinatorial library of potential agonists inside a eukaryotic cell and then directly selecting for an agonist of a target molecule. Some methods involve co-culturing a cell expressing a combinatorial library of potential agonists and a second cell, and then selecting agents that modulate a phenotype of or a desired cellular response in the second cell. Preferably, the agonists are antibodies introduced into and expressed in the starting cells, such as agonist anti-EpoR, anti-TpoR, or G-CSFR antibodies. Also disclosed are methods for selecting from combinatorial antibody libraries bispecific antibodies that can regulate cell phenotypes. |
申请公布号 |
AU2013309272(B2) |
申请公布日期 |
2017.03.09 |
申请号 |
AU20130309272 |
申请日期 |
2013.08.16 |
申请人 |
Wilson, Ian;Lerner, Richard;Zhang, Hongkai;The Scripps Research Institute |
发明人 |
Zhang, Hongkai;Wilson, Ian A.;Lerner, Richard A. |
分类号 |
C40B50/06;C12N5/00;C12P21/06 |
主分类号 |
C40B50/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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