INVERTEBRATE MICRORNAS

摘要

This invention provides plants having resistance to invertebrate pests. More specifically, this invention discloses a non-natural transgenic plant cell expressing at least one invertebrate miRNA in planta for suppression of a target gene of an invertebrate pest or of a symbiont associated with the invertebrate pest. Also provided are recombinant DNA constructs for expression of at least one invertebrate miRNA in planta, a non-natural transgenic plant containing the non-natural transgenic plant cell of this invention, a non-natural transgenic plant grown from the non-natural transgenic plant cell of this invention, and non-natural transgenic seed produced by the non-natural transgenic plants, as well as commodity products produced from a non-natural transgenic plant cell, plant, or seed of this invention. This invention further provides a method of suppressing at least one target gene of an invertebrate pest of a plant or of a symbiont associated with the invertebrate, including providing a plant including the non-natural transgenic plant cell of this invention, wherein the invertebrate is the invertebrate pest, the recombinant DNA is transcribed in the non-natural transgenic plant cell to the recombinant miRNA precursor, and when the invertebrate pest ingests the recombinant miRNA precursor, the at least one target gene is suppressed.

主权项

1. A non-natural plant having resistance to an invertebrate pest that ingests RNA from said plant, wherein
said non-natural plant comprises a transgenic plant cell having in its genome a recombinant DNA construct that is transcribed in said transgenic plant cell to a recombinant miRNA precursor; and said recombinant miRNA precursor comprises a single strand of RNA that folds into the secondary structure of an invertebrate miRNA precursor and that comprises at least one stem-loop that is processed to a mature miRNA; and said mature miRNA suppresses expression of at least one target gene of said invertebrate pest or of a symbiont associated with said invertebrate pest, thereby conferring on said non-natural plant resistance to said invertebrate pest.