| 发明名称 |
ISOFORM ENRICHED ANTIBODY PREPARATION AND METHOD FOR OBTAINING IT |
| 摘要 |
Herein is reported a method for producing an antibody preparation comprising the steps of a) applying a buffered solution comprising different isoforms of an antibody to a cation exchange chromatography material, b) applying a first solution with a first conductivity to the cation exchange chromatography material, whereby the antibody isoforms remain bound to the cation exchange chromatography material, and c) applying a second solution with a second conductivity to the cation exchange chromatography material and thereby obtaining the antibody preparation, whereby the conductivity of the second solution exceeds the conductivity of the first solution by not more than 10%. |
| 申请公布号 |
US2017066814(A1) |
申请公布日期 |
2017.03.09 |
| 申请号 |
US201514934866 |
申请日期 |
2015.11.06 |
| 申请人 |
Hoffmann-La Roche Inc. |
发明人 |
Falkenstein Roberto;Schwendner Klaus;Spensberger Bernhard |
| 分类号 |
C07K16/00;C07K16/32;C07K16/28;C07K1/18 |
主分类号 |
C07K16/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for producing an antibody preparation comprising the following steps:
a) applying a buffered solution comprising different isoforms of an antibody to a cation exchange chromatography material, b) applying a first solution with a first conductivity to the cation exchange chromatography material, whereby the antibody isoforms remain adsorbed to the cation exchange chromatography material, c) applying a second solution with a second conductivity to the cation exchange chromatography material and thereby obtaining the antibody preparation, whereby the conductivity of the second solution exceeds the conductivity of the first solution by not more than 50%. |
| 地址 |
Little Falls NJ US |
