| 发明名称 |
BIOCATALYSTS AND METHODS FOR THE SYNTHESIS OF ARMODAFINIL |
| 摘要 |
The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (−)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess. |
| 申请公布号 |
US2017067036(A1) |
申请公布日期 |
2017.03.09 |
| 申请号 |
US201615352970 |
申请日期 |
2016.11.16 |
| 申请人 |
CODEXIS, INC. |
发明人 |
Ang Ee Lui;Alvizo Oscar;Behrouzian Behnaz;Clay Michael D.;Collier Steven J.;Eberhard Ellen D.;Fan Fu;Song Shiwei;Smith Derek J.;Widegren Magnus;Wilson Robert;Xu Junye;Zhu Jun |
| 分类号 |
C12N9/02;C12P11/00 |
主分类号 |
C12N9/02 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A non-naturally occurring polynucleotide encoding a polypeptide having cyclohexanone monooxygenase (CHMO) activity wherein the amino acid sequence of the polypeptide has at least 90% sequence identity to SEQ ID NO:2, and one or more amino acid substitutions at one or more positions in SEQ ID NO: 2 selected from the group consisting of 277, 281, 326, 433, or 435. |
| 地址 |
Redwood City CA US |
