NOVEL INSULIN ANALOGS AND USE THEREOF

摘要

The present invention relates to a novel insulin analog, and more specifically, to an insulin analog with an improved in vitro effect compared with native insulin, a nucleic acid encoding the same, an expression vector including the nucleic acid, a transformant introduced with the expression vector, a method of producing the insulin analog from the transformant, a pharmaceutical composition for treating diabetes containing the insulin analog as an active ingredient, and a method for treating diabetes using the insulin analog or the pharmaceutical composition.

主权项

1: An insulin analog peptide comprising an A-chain of SEQ ID NO: 3 indicated in the following General Formula 1 and a B-chain of SEQ ID NO: 4 indicated in the following General Formula 2:(General Formula 1)(SEQ ID NO: 3)Xaa1-Ile-Val-Glu-Xaa2-Cys-Cys-Thr-Ser-Ile-Cys-Xaa3-Leu-Xaa4-Gln-Xaa5-Glu-Asn-Xaa6-Cys-Xaa7 wherein Xaa1 is alanine, glycine, glutamine, histidine, glutamic acid, or asparagine; Xaa2 is alanine, glutamic acid, glutamine, histidine, or asparagine; Xaa3 is alanine, serine, glutamine, glutamic acid, histidine, or asparagine; Xaa4 is alanine, tyrosine, glutamic acid, histidine, lysine, aspartic acid, or asparagine; Xaa5 is alanine, leucine, tyrosine, histidine, glutamic acid, or asparagine; Xaa6 is alanine, tyrosine, serine, glutamic acid, histidine, or asparagine; and Xaa7 is asparagine, glycine, histidine, or alanine; and(General Formula 2)(SEQ ID NO: 4)Phe-Val-Asn-Gln-His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Xaa8-Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe-Xaa9-Tyr-Xaa10-Xaa11-Lys-Thr wherein Xaa8 is tyrosine, glutamic acid, or aspartic acid, or is absent; Xaa9 is phenylalanine, or is absent; Xaa10 is threonine, or is absent; and Xaa11 is proline, glutamic acid, or aspartic acid, or is absent;wherein the insulin analog peptide is not a peptide comprising the A-chain of SEQ ID NO: 1 and the B-chain of SEQ ID NO: 2.