TREATMENT OF INTERVERTEBRAL DISC DEGENERATION USING HUMAN UMBILICAL CORD TISSUE-DERIVED CELLS

摘要

Methods for treating a patient having a disease or condition related to IVD degeneration are provided. The methods comprise administering cells obtained from human umbilical cord tissue, or administering pharmaceutical compositions comprising such cells or prepared from such cells and optionally a hydrogel. In some embodiments, administering the cells promotes repair and regeneration of degenerated IVD tissue in the patient. Pharmaceutical compositions for use in the inventive methods, as well as kits for practicing the methods are also provided.

主权项

1. A method treating disc degeneration in a subject comprising administering a pharmaceutical composition comprising a mixture of a hydrogel and an isolated homogenous population of cells obtained from human umbilical cord tissue into an intervertebral disc,
wherein the umbilical cord tissue is substantially free of blood, wherein the hydrogel comprises fibrinogen and thrombin, and wherein the isolated homogenous population of cells is capable of self-renewal and expansion in culture, has the potential to differentiate and has the following characteristics: does not express CD31, CD34, CD117, telomerase and HLA-DR; expresses chemokine receptor ligand 3, and/or granulocyte chemotactic protein; expresses CD10, CD13, CD44, CD73, PDGFr-alpha and CD90; and expresses, relative to a human fibroblast, mesenchymal stem cell, or iliac crest bone marrow cell, increased levels of interleukin 8 and reticulon 1.