| 发明名称 |
COMPOSITIONS AND METHODS FOR DETECTING HUANGLONGBING |
| 摘要 |
The present invention features compositions and methods for detecting Huanglongbing (HLB) in citrus trees and insects. In one aspect, the invention provides a method of detecting a Huanglongbing (HLB) infection in a citrus grove involving obtaining an extract from a biological sample derived from a citrus grove, contacting the extract with forward and reverse primers that specifically bind a Candidutus nucleic acid molecule in the presence of a nicking enzyme, dNTPs, and a polymerase under conditions permissive for the isothermal amplification of the nucleic acid molecule; and detecting a Candidutus amplicon in the extract. |
| 申请公布号 |
US2017114394(A1) |
申请公布日期 |
2017.04.27 |
| 申请号 |
US201515306750 |
申请日期 |
2015.04.28 |
| 申请人 |
ENVIROLOGIX INC. |
发明人 |
PARKER BRECK;TAPLEY SUSAN;LAMPTON PAULA |
| 分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of detecting a Huanglongbing (HLB) infection in a biological sample, the method comprising
obtaining an extract from a biological sample, contacting the extract with forward and reverse primers that specifically bind a Candidutus nucleic acid molecule in the presence of a nicking enzyme, dNTPs, and a polymerase under conditions permissive for the isothermal amplification of the nucleic acid molecule; and detecting a Candidutus amplicon in the extract, wherein the presence of the Candidutus amplicon detects an HLB infection in the sample and failure to detect the amplicon indicates the absence of an HLB infection in the sample. |
| 地址 |
PORTLAND ME US |
