MASS SPECTROMETRIC DETERMINATION OF COOKSON-DERIVATIZED, NON-METABOLIZED VITAMIN D

摘要

The invention relates to the detection of vitamin D. In a particular aspect, the invention relates to methods for detecting derivatized vitamin D by mass spectrometry.

主权项

1. A method for determining the amount of non-metabolized vitamin D in a sample by tandem mass spectrometry, the method comprising the steps of:
(i) purifying the sample by liquid-liquid extraction; (ii) subjecting the sample to turbulent flow liquid chromatography (TFLC); (iii) subjecting Cookson-type-derivatized non-metabolized vitamin D from the sample to an ionization source to generate one or more precursor ions detectable by mass spectrometry; (iv) fragmenting at least one of said precursor ions to generate one or more fragment ions detectable by mass spectrometry; (v) determining the amount of one or more precursor and fragment ions by mass spectrometry; and (vi) relating the amount of ions determined in step (iii) to the amount of a non-metabolized vitamin D in the sample.