METHODS AND KITS FOR NUCLEIC ACID ISOLATION

摘要

The current teachings disclose methods and kits for increasing the amount of nucleic acid that can be recovered from magnetic beads using elution solutions with high ionic strength.

主权项

1. A method for isolating nucleic acid from a biological sample comprising:
combining a biological sample, a nucleic acid extraction reagent, and magnetic particles in a vessel; incubating the vessel under conditions suitable for binding the nucleic acid to the magnetic particles; placing the vessel in proximity to a magnetic field to allow the magnetic particles to migrate in the magnetic field to at least one surface of the vessel; substantially removing the nucleic acid extraction reagent from the vessel; removing the magnetic field from the proximity of the vessel; resuspending the magnetic beads in an elution solution comprising at least 20 mM sodium chloride (NaCl) to form an elution composition; incubating the elution composition under conditions suitable for releasing at least some of the nucleic acid from the magnetic particles into the solution; placing the vessel in proximity to a magnetic field to allow the magnetic particles to migrate in the magnetic field to at least one surface of the vessel; and removing at least some of the elution solution comprising at least some of the released nucleic acid from the vessel, thereby isolating nucleic acid from the sample.