BINDING PARTNERS SPECIFIC FOR VITAMIN D EPIMERS

摘要

Antibodies are raised against compounds of Formula (I) where Z is an immunogenic carrier. These compounds are capable of binding specifically to corresponding antibodies. The antibodies are specific for epimers of vitamin D. Antibodies in accordance with the principles described herein may be employed to minimize or eliminate 3-epimer cross-reactivity in assays for non-epimeric forms of vitamin D analytes. Over-estimation of total non-epimeric vitamin D analyte caused by the cross-reactivity of 3-epimer vitamin D with an antibody for vitamin D analyte can be substantially avoided employing, as blocking agents, antibodies prepared against immunogens that are a compound of the Formula (I) wherein Z is an immunogenic carrier.;

主权项

1. A binding partner prepared against a compound of the formula:
(R1)p-(L)q-Z wherein  or H, wherein at least one R1 is not H, Y is O, S, CR, or NR4, X is —O—(CH2)n—C(O)—, —(CH2)w—C(O)—, —(CH2)w—C(O)—(CH2)x—C(O)—, —(CH2)w—C(O)—NH(CH2)y—C(O)—, or —NR3—C(O)—, R is independently H or alkyl, R2 is independently H or alkyl, R3, R4, R5 and R6 are independently H or alkyl, or R4 and R5 may be taken together to form a bond, or R5 and R6 may be taken together to form a bond, or R and R5 may be taken together to form a bond, R11 is H, alkyl, or acyl, n is an integer from 0 to 10, w is an integer from 1 to 10, x is an integer from 1 to 10, y is an integer from 1 to 10, p is an integer from 1 to 10, L is a linking group, q is 0 or 1, and Z is an immunogenic carrier; or raised against mixtures of two or more of the compounds.