| 发明名称 |
Mutant-Type Glucose Dehydrogenase and Use Thereof |
| 摘要 |
The present invention provides a mutant-type glucose dehydrogenase having glucose dehydrogenase activity and having decreased reactivity with xylose, wherein said mutant-type glucose dehydrogenase comprises a mutant-type α-subunit comprising an amino acid sequence of 520 to 550 amino acids comprising an amino acid sequence of 520 to 550 amino acids comprising SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24 and SEQ ID NO: 25 in this order from N-terminus to C-terminus, except that one or more amino acid residue(s) selected from the group consisting of the glycine at position 10 in SEQ ID NO: 23, the histidine at position 4 in SEQ ID NO: 24, and the asparagine at position 4 in SEQ ID NO: 25 is/are substituted with another/other amino acid(s). |
| 申请公布号 |
US2017121751(A1) |
申请公布日期 |
2017.05.04 |
| 申请号 |
US201615337630 |
申请日期 |
2016.10.28 |
| 申请人 |
ARKRAY, Inc. ;Ultizyme International Ltd. |
发明人 |
Kojima Katsuhiro;Mori Kazushige |
| 分类号 |
C12Q1/00;C12N9/04 |
主分类号 |
C12Q1/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A mutant-type glucose dehydrogenase having glucose dehydrogenase activity and decreased reactivity with xylose,
wherein said mutant-type glucose dehydrogenase comprises a mutant-type α-subunit comprising an amino acid sequence of 520 to 550 amino acids comprising SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24 and SEQ ID NO: 25 in this order from N-terminus to C-terminus, except that one or more amino acid residue(s) selected from the group consisting of the glycine at position 10 in SEQ ID NO: 23, the histidine at position 4 in SEQ ID NO: 24, and the asparagine at position 4 in SEQ ID NO: 25 is/are substituted with another/other amino acid(s). |
| 地址 |
Kyoto JP |
