METHOD OF PRETREATMENT OF BIOLOGICAL SAMPLES FOR AN ANALYTE-STAINING ASSAY METHOD

摘要

Disclosed are methods for treating a biological sample for an analyte-staining assay. These methods are conducted with the use of a single aqueous multi-functional composition for the purposes of performing the steps of deparaffinization, rehydration, antigen retrieval and endogenous enzyme blocking simultaneously optionally at an elevated temperature. These methods are alternatively conducted with use of a single aqueous multi-functional composition that contains a thermostable protease for the purpose of deparaffinization, rehydration, antigen retrieval and endogenous enzyme blocking at an elevated temperature.

主权项

1. A method for treating a biological sample for conducting an analyte-staining assay, comprising:
obtaining a biological sample, wherein
the biological sample is fixed by a fixative and affixed on a solid substrate; contacting the biological sample with one aqueous composition, wherein
the aqueous composition comprises a buffered condition and one or more endogenous enzyme inhibitor wherein
the aqueous composition is capable of hydrating the sample, retrieving a denatured target molecule in the biological sample caused by the fixative, and inhibiting an endogenous enzyme that is naturally occurred in the sample; and removing the contact from the sample, wherein
the treated sample contains enhanced condition for the analyte-staining assays.