Methods and compositions for modulating peripheral immune function

摘要

Disclosed herein are cell preparations useful for modulating various peripheral immune functions, methods for making said cell preparations, and methods for their use.

主权项

1. A method for modulating production of one or more cytokines by a T-lymphocyte (T-cell) or a monocyte; the method comprising:
contacting the T-cell or monocyte with an effective amount of SB623MASC-derived immune modulatory cells; wherein said contacting results in stimulation of the production of one or more anti-inflammatory cytokines and/or inhibition of production of one or more pro-inflammatory cytokines by the T-cell or the monocyte; further wherein said SB623MASC-derived immune modulatory cells are obtained by (a) providing a culture of marrow adherent stromal cells (MASCs); (b) contacting the cell culture of step (a) with a polynucleotide comprising sequences encoding the Notch intracellular domain (NICD) wherein said polynucleotide dos not encode a full-length Notch protein; (c) selecting cells that comprise the polynucleotide of step (b); and (d) further culturing the selected cells of step (c) in the absence of selection for the polynucleotide, such that said marrow adherent stromal cells are induced to form SB623MASC-derived immune modulatory cells by expression of the NICD.