| 主权项 |
1. A method of imaging reactive oxygen species, reactive nitrogen species, or both in a subject, the method comprising:
administering to the subject an optical sensor conjugate comprising a fluorogenic small molecule, a particle or polymer, and a linker that connects the small molecule to the particle or polymer, for a time sufficient for the small molecule to react with the reactive oxygen or reactive nitrogen, or both, thereby releasing the small molecule from the optical sensor conjugate and becoming fluorescent; and obtaining a fluorescence image of the small molecule in the subject, thereby imaging the subject,
wherein the small molecule with the linker comprises a compound of Formula I:wherein:
Z is the linker and comprises C3-20 alkyl, wherein any of the carbons in C3-20 alkyl can be replaced with —C(O)—, C(O)O—, —C(O)NRA—, —C(NH)—, oxygen, sulfur, —SO2—, —NRASO2— —NRA—; X is selected from N3, C(O)Cl, and C(O)ORA; Y is selected from O, S, Se, Te, N(RA), and C(Me)2; R1 is selected from H, C1-6 alkyl, ORA, and NRCRD, wherein said C1-6 alkyl is optionally substituted by ORA or NRCRD; or 2 or 3 R1 adjacent to each other and together with the C atoms to which they are attached form 1or 2 heterocycloalkyl, optionally substituted by 1, 2, 3, or 4 substituents independently selected from H, OH, C1-6 alkyl, ORA, C(O)RB, C(O)NRCRD, and C(O)ORA; RA, RB, RC and RD are independently selected from H, C1-6 alkyl, and succinimidyl; and m is 1, 2, 3, or 4. |
