| 主权项 |
1. A process for the preparation of a bioconjugate, the process comprising the step of reacting a reactive group Q1 of a linker-conjugate with a functional group F1 of a glycoprotein (B) under condition such that the reactive group Q1 is reacted with the functional group F1 of the glycoprotein to covalently link the glycoprotein to the linker-conjugate,
wherein the linker-conjugate is a compound comprising a group according to formula (1) covalently linked to an alpha-end at one side of the formula (1) and an omega-end at the other side of the formula (1), the alpha-end comprising the reactive group Q1 capable of reacting with the functional group F1 present on the glycoprotein (B) and the omega-end comprising a target molecule (D) selected from the group consisting of an active substance, a reporter molecule, a polymer, a solid surface, a hydrogel, a nanoparticle, a microparticle and a biomolecule, wherein: the wavy lines indicate covalent linkages to the alpha end and the omega end, a is 0 or 1; and R1 is selected from the group consisting of hydrogen, C1-C24 alkyl groups, C3-C24 cycloalkyl groups, C2-C24 (hetero)aryl groups, C3-C24 alkyl(hetero)aryl groups and C3-C24 (hetero)arylalkyl groups, the C1-C24 alkyl groups, C3-C24 cycloalkyl groups, C2-C24 (hetero)aryl groups, C3-C24 alkyl(hetero)aryl groups and C3-C24 (hetero)arylalkyl groups optionally substituted and optionally interrupted by one or more heteroatoms selected from O, S and NR3 wherein R3 is independently selected from the group consisting of hydrogen and C1-C4 alkyl groups, or R1 is a second target molecule D, wherein the second target molecule is optionally connected to N via a spacer moiety; and wherein Q1 is a (hetero)cyclooctynyl group or a bicycle[6.1.0]non-4-yn-9yl] group, and F1 is an azide group. |
