Variant <i>Trichoderma reesei </i>endoglucanases

摘要

The present invention provides T. reesei endoglucanase I variants exhibiting improved cellulase and/or xylanase activity, in particular at 30° C. and/or pH 6. These variants are particularly useful in industrial processes comprising simultaneous degradation of cellulosic biomass into monomeric sugars and fermentation of said sugars, for example to produce ethanol.

主权项

1. An endoglucanase I variant comprising a sequence (i) having at least 96% identity to the full length amino acid sequence set forth in SEQ ID NO: 3 and (ii) comprising at least one substitution at position corresponding to residue E117, D241, T7 or M91 of SEQ ID NO: 3, wherein said variant exhibits an improved cellulase and/or xylanase activity compared to Trichoderma reesei endoglucanase I of SEQ ID NO: 2.