Compositions and methods including recombinant B lymphocyte cell line including an exogenously incorporated nucleic acid expressing an exogenous membrane immunoglobulin reactive to a first antigen and including an endogenous gene expressing an endogenous secreted immunoglobulin reactive to a second antigen

摘要

Compositions and methods are disclosed herein for producing one or more immunoglobulins in an isolated B lymphocyte cell line. An isolated cell line includes an isolated B lymphocyte cell line capable of expressing at least one exogenously incorporated membrane immunoglobulin reactive to a first antigen and at least one endogenous secreted immunoglobulin reactive to a second antigen.

主权项

1. A method for producing an immunoglobulin in an isolated B lymphocyte cell line comprising:
isolating from a vertebrate subject exposed to at least one first antigen, a B lymphocyte cell, and growing the B lymphocyte in vitro to obtain a B lymphocyte cell line expressing at least one endogenous secreted immunoglobulin reactive to the at least one first antigen; introducing into the isolated B lymphocyte cell line at least one exogenous membrane immunoglobulin reactive to at least one second antigen to produce a recombinant B lymphocyte cell line; selecting the isolated B lymphocyte cell line expressing the membrane immunoglobulin reactive to the at least one second antigen and expressing the at least one endogenous secreted immunoglobulin reactive to the at least one first antigen.