Biomarkers of oxidative stress

摘要

The present invention relates to a method for detecting cardiovascular oxidative stress in an individual, comprising detecting in a blood sample from the individual modification of a cysteine at position 45 of the β1-subunit of the human erythrocyte ATP-dependent Na+K+ pump protein or of an equivalent cysteine in a homolog or variant thereof. The invention further relates to a kit for detecting cardiovascular oxidative stress in an individual, the kit comprising at least one agent for detecting the presence of a modification in a cysteine at position 45 of the β1-subunit of the human erythrocyte ATP-dependent Na+K+ pump protein or of an equivalent cysteine in a homolog or variant thereof, wherein said modification is a result of oxidation.

主权项

1. A method of detecting glutathionylation of β1 subunit of human erythrocyte ATP-dependent Na+K+ pump protein in an individual, said method comprising
a) obtaining a blood sample from the individual, b) contacting erythrocyte membranes prepared from said sample with a first antibody specific for glutathione bound to a cysteine residue at position 45 within SEQ ID NO: 1 of the β1 subunit of the human erythrocyte ATP-dependent Na+K+ pump protein and a second antibody specific for the β1 subunit of the human erythrocyte ATP-dependent Na+K+ pump protein comprising the cysteine residue at position 45 within SEQ ID NO: 1, and c) detecting the binding between the first antibody and glutathione bound to a cysteine residue at position 45 within SEQ ID NO: 1 of the β1 subunit of the human erythrocyte ATP-dependent Na+K+ pump protein in the blood sample from the individual and the binding between the second antibody and the β1 subunit of the human erythrocyte ATP-dependent Na+K+ pump protein comprising the cysteine residue at position 45 within SEQ ID NO: 1 in the blood sample from the individual.