Recombinant protein body-inducing polypeptides

摘要

Polypeptide sequences for inducing recombinant protein bodies are described. The sequences comprise a polyproline II (PPII) structure and/or a proline-rich sequence between two cysteine residues on either end. Recombinant protein bodies are useful for protein production because they allow for simple and efficient purification of high quantities of recombinant protein. In addition, other methods of using recombinant protein bodies, for example, in vaccination and food products, are also described.

主权项

1. A recombinant protein body-inducing polypeptide sequence (PBIS) comprising a polyproline II (PPII) structure that is at least 36 amino acids in length and has an N-terminus and a C-terminus wherein
(i) the PPII structure is located between at least two cysteines at the N-terminus and at least two cysteines at the C-terminus; (ii) no more than 10% of the amino acids in the PPII structure are lysine or arginine; (iii) the PPII structure does not contain the sequence (PPPVHL)6 showing the sequence set forth in SEQ ID NO:115; and (iv) the PPII structure is selected from the group consisting of a non-amphipathic PPII structure, an amphipathic and negatively charged PPII structure, and an amphipathic and non-charged PPII structure, wherein the non-amphipathic PPII structure, is selected from the group consisting of a sequence comprising at least 6 repeats of the sequence set forth in SEQ ID NO: 121, a sequence comprising at least 6 repeats of the sequence set forth in SEQ ID NO: 122, a sequence comprising at least 6 repeats of the sequence set forth in SEQ ID NO: 123, a sequence comprising at least 6 repeats of the sequence set forth in SEQ ID NO: 120 wherein the X residues are proline, and a sequence comprising at least 6 repeats of the sequence set forth in SEQ ID NO: 129; wherein the amphipathic and negatively charged PPII structure is selected from the group consisting of a sequence comprising at least 6 repeats of the sequence set forth in SEQ ID NO: 124 and a sequence comprising at least 6 repeats of the sequence set forth in SEQ ID NO: 125; and wherein the non-charged PPII structure is selected from the group consisting of a sequence comprising at least 6 repeats of the sequence set forth in SEQ ID NO: 126, a sequence comprising at least 6 repeats of the sequence set forth in SEQ ID NO: 127, and a sequence comprising at least 6 repeats of the sequence set forth in SEQ ID NO: 128.