Generation of functional and durable endothelial cells from human amniotic fluid-derived cells

摘要

This disclosure is directed to methods for reproducibly generating substantial amounts of endothelial cells from amniotic cells. The endothelial cells generated in accordance with the present methodology, as well as therapeutic methods utilizing these cells, are also disclosed.

主权项

1. A method of generating endothelial cells, comprising
obtaining amniotic cells; and culturing the amniotic cells for at least 21 days under conditions where transcription factors ETV2, FLI1 and ERG are expressed in the amniotic cells in the presence of a TGFβ signaling inhibitor, wherein said amniotic cells express ETV2 for the first 13-15 days, said TGFβ signaling inhibitor is present for the first 20-21 days, and FLI1 and ERG are constitutively expressed, thereby obtaining endothelial cells.