Neutralizing Antibodies and Methods of Use Thereof

摘要

This invention provides monoclonal antibodies that recognize the Toll-like Receptor 4/MD-2 receptor complex, and monoclonal antibodies that recognize the TLR4/MD2 complex as well as TLR4 when not complexed with MD-2. The invention further provides methods of using the monoclonal antibodies as therapeutics. This invention also provides soluble chimeric proteins, methods of expressing and purifying soluble chimeric proteins, and methods of using soluble chimeric proteins as therapeutics, in screening assays and in the production of antibodies.

主权项

1. An antibody that immunospecifically binds a Toll-like receptor 4 (TLR4)/MD-2 complex, wherein the antibody exhibits greater than 50% inhibition of lipopolysaccharide (LPS)-induced IL-8 production in human TLR4/MD-2 transfected HEK293 cells at a concentration of 10 μg/ml.