NOVEL ALTERNATIVELY SPLICED TRANSCRIPTS AND USES THEREOF FOR IMPROVEMENT OF AGRONOMIC CHARACTERISTICS IN CROP PLANTS

摘要

Computational analysis of hundreds of RNA-seq libraries enabled the identification of novel transcripts in maize. The novel transcripts are provided herein, as are recombinant DNA constructs comprising such, transgenic plants or cell thereof comprising the recombinant DNA constructs, and methods for generating transgenic seed and plants with improved agronomic characteristics.

主权项

1. A recombinant DNA construct comprising a polynucleotide operably linked to at least one regulatory sequence wherein said polynucleotide comprises:
a. a nucleic acid sequence of at least 95% sequence identity, based on the Clustal V method of alignment, when compared to any of SEQ ID NOs:1-157,066 and 198,539-222,468; b. a nucleic acid sequence encoding an amino acid sequence of at least 95% sequence identity, based on the Clustal V method of alignment, when compared to any of SEQ ID NOs:157,067-198,538 and 222,469-228,453; or c. a nucleic acid sequence that is transcribed into an RNA molecule that suppresses the level of an endogenous polypeptide having an amino acid sequence of at least 95% sequence identity, based on the Clustal V method of alignment, when compared to any of SEQ ID NOs:157,067-198,538 and 222,469-228,453.