SMALL MOLECULE DETECTION AND LOCALIZATION METHOD BASED ON MALDI IMAGING MASS SPECTROMETRY

摘要

Disclosed is a angiogenesis inhibition determining method using MALDI mass spectrometry, and more particularly, relate to a method for detecting whether small molecules are bound with a target protein and for measuring a binding distribution between the small molecules and the target protein by comparing a result of MALDI mass spectrometry with a result of immunofluorescence of the small molecules, which are used as drugs, for the target protein, and for determining as angiogenesis is inhibited in a portion overlapping with a portion where the drug small molecules are present after the MALDI mass spectrometry in the cell or in the biosample including organelles and a portion where the target protein is present after immunofluorescence, as well as for detecting presence or absence and a distribution state of small molecules used as drugs in a sample by using MALDI mass spectrometry.

主权项

1. An angiogenesis inhibition determining method using MALDI mass spectrometry, the method comprising:
preparing a sample; treating a drug in the sample; performing MALDI mass spectrometry for the drug-treated sample; detecting a target protein for the drug in the prepared sample by immunofluorescence; comparing a result of the MALD mass spectrometry with a result of immunofluorescence for the target protein; and determining that angiogenesis is inhibited in a portion overlapping with a portion where the small molecules are present after the MALDI mass spectrometry and a portion where the target protein is present after immunofluorescence.