| 发明名称 |
METHOD AND KIT FOR QUANTIFYING CARDIOLIPIN |
| 摘要 |
The inventions disclosed herein are a method for quantifying cardiolipin in a sample, comprising the steps of: (1) treating the sample with phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase and (2) measuring the fluorescence intensity, absorbance, or luminescence intensity of a compound generated in step (1) to quantify cardiolipin using a calibration curve obtained beforehand; and a kit for quantifying cardiolipin comprising phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase. |
| 申请公布号 |
US2017114387(A1) |
申请公布日期 |
2017.04.27 |
| 申请号 |
US201515301133 |
申请日期 |
2015.03.17 |
| 申请人 |
SHIGA UNIVERSITY OF MEDICAL SCIENCE |
发明人 |
MORITA Shin-ya |
| 分类号 |
C12Q1/28;G01N21/64;C12Q1/32;C12Q1/44;C12Q1/48 |
主分类号 |
C12Q1/28 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for quantifying cardiolipin in a sample, comprising the steps of:
(1) treating the sample with phospholipase D, glycerol kinase, glycerol-3-phosphate oxidase, and peroxidase and (2) measuring the fluorescence intensity, absorbance, or luminescence intensity of a compound generated in step (1) to quantify cardiolipin using a calibration curve obtained beforehand. |
| 地址 |
Otsu-shi, SHIGA JP |
