APTAMERS WITH BINDING AFFINITY TO NOROVIRUS

摘要

The instant disclosure provides norovirus-binding aptamers, compositions comprising such aptamers, and methods of using and producing such aptamers. The aptamers are useful, for example, for detecting the presence of norovirus in test samples, for capturing and/or concentrating norovirus from test samples, for evaluating the efficacy of therapeutic agents in patients diagnosed with a norovirus infection, and for evaluating the efficacy of norovirus vaccines.

主权项

1. A method of detecting the presence of at least one norovirus strain in a test sample, the method comprising:
(a) contacting said test sample with a norovirus-binding aptamer comprising a norovirus-binding motif and/or any one of SEQ ID NOS: 1-78 and 176-181 or variants thereof having at least 90% sequence identity and having norovirus-binding activity; and (b) detecting the presence of said norovirus-binding aptamer bound to norovirus in said test sample, wherein detection of bound aptamer indicates the presence of at least one norovirus strain.