| 主权项 |
1. A method for an extended periodic harvest comprising
establishing a cell culture by inoculating a bioreactor with mammalian cells expressing a recombinant protein, maintaining the cell culture by perfusing fresh cell culture medium into the bioreactor, passing the cell culture through a filter and collecting a permeate, wherein a null permeate is initially collected until a first predetermined parameter is reached, at which time a harvest permeate is collected for a predetermined time, this is followed by alternately collecting a null permeate until a second predetermined parameter is reached, then collecting a harvest permeate for a predetermined time, wherein the alternating collection of null permeate and harvest permeate continues until the cell culture is terminated. |
